This tutorial will serve as a guideline for how to go about analyzing RNA sequencing data when a reference genome is available. We will be going through quality control of the reads, alignment of the reads to the reference genome, conversion of the files to raw counts, analysis of the counts wit ... Jan 23, 2018 · The 2+4 alignment process you just finished is not the polar alignment, but the pointing model for the GOTO system. For visual observers you’re set now, and can stop following the tutorial here. If you intend to do imaging, the next steps will cover the polar alignment process to dial in that last bit of precision for long exposure imaging.